Promoter for a Brassica napus ribulose bisphosphate carboxylase/oxygenase small subunit gene binds multiple nuclear factors and contains a negative-strand open reading frame encoding a putative transmembrane protein

• Published in: Plant molecular biology Vol. 16; no. 6; p. 955
• Main Authors: Nantel, A.M. (Laval Univ., Laval, Quebec (Canada). Dept. de Biochimie), Lafleur, F, Boivin, R, Baszczynski, C.L, Bellemare, G
• Format: Journal Article
• Language: English
• Published: Netherlands 01.06.1991
• Subjects: Amino Acid Sequence; Base Sequence; Brassica - enzymology; Brassica - genetics; BRASSICA NAPUS; Cloning, Molecular; CODE GENETIQUE; CODIGO GENETICO; DNA-Binding Proteins - metabolism; GENE; Gene Expression Regulation - physiology; GENES; Genes, Plant - genetics; GENETIC CODE; Membrane Proteins - genetics; Molecular Sequence Data; Multigene Family - genetics; Nucleic Acid Conformation; Open Reading Frames - genetics; Promoter Regions, Genetic - genetics; PROTEINAS; PROTEINE; PROTEINS; Ribulose-Bisphosphate Carboxylase - genetics; Sequence Homology, Nucleic Acid
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/BF00016068

Using a fractionated genomic bank, we have cloned and characterized a Brassica napus gene (rbcSF1) encoding the small sub-unit of ribulose 1,5-bisphosphate carboxylase. The promoter of this gene contains a 29 bp direct repeat capable of forming a single or a double hairpin loop, and three elements that are recognized by leaf nuclear proteins in vitro. The most upstream are the S-box, a small A/T-rich sequence between -516 and -512, and the F-box between -492 and -475. Finally, we have also observed binding to the G-box, a regulatory element common to numerous plant promoters. The promoter of rbcSF1 also has a 113 amino acids open reading frame (ORF113) in the non-coding strand. When used to probe a northern blot of leaf RNA, this ORF hybridizes to a 1.5 kb transcript. The protein encoded by ORF113 contains a transmembrane domain.