Rapid UPLC–ESI–MS/MS method for the analysis of isoflavonoids and other phenylpropanoids

• Published in: Journal of food composition and analysis Vol. 26; no. 1-2; pp. 36 - 42
• Main Authors: Prokudina, E.A., Havlíček, L., Al-Maharik, N., Lapčík, O., Strnad, M., Gruz, J.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Inc 01.05.2012; Elsevier
• Subjects: Biological and medical sciences; Flavonoids; Food analysis; Food composition; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; Isoflavones; Isoflavonoids; Methods of analysis, processing and quality control, regulation, standards; Mung bean (Vigna radiata); Tandem mass spectrometry (MS/MS); Ultra-performance liquid chromatography (UPLC); Ultra-performance liquid chromatography (UPLC); Tandem mass spectrometry (MS/MS); Flavonoids; Mung bean (Vigna radiata); Isoflavonoids; Food composition; Food analysis; Isoflavones; Flavonoid; Mung bean; Phytoestrogen; Polyphenol; Dicotyledones; Angiospermae; Food; Isoflavone; Vigna radiata; Liquid chromatography; Method; Leguminosae; Analysis; Mass spectrometry MS/MS; Spermatophyta; Performance; Mass spectrometry
• ISSN: 0889-1575; 1096-0481
• DOI: 10.1016/j.jfca.2011.12.001

► A rapid UPLC–MS/MS method for the quantification of isoflavonoids was developed. ► 26 compounds were analyzed simultaneously in a single 17min run. ► 14 compounds out of the set of 26 analytes were quantified in mung bean sprouts. ► No preconcentration was needed to quantify the analytes in plant material. ► Organic solvent consumption was substantially reduced compared to HPLC. UPLC–ESI–MS/MS method was developed for the quantitative and qualitative analysis of 26 phenolic compounds in plant material, including 15 isoflavonoids (daidzein, genistein, isoformononetin, formononetin, prunetin, biochanin A, daidzin, genistin, ononin, sissotrin, osajin, pomiferin), 5 flavones (apigenin, kaempferol, quercetin, quercetin-3-glucoside, rutin), 4 flavanones (hesperetin, naringenin, naringenin-7-glucoside, naringin), a coumestan (coumestrol) and a coumarine (scopoletin). The analytes were detected in a multiple reaction monitoring mode and quantified using internal standards [13C3]daidzin, [2H6]naringenin and [2H4]genistein. Limits of detection were in the range 0.001–10pmolinjection−1. The precision of the method (relative standard deviation) ranged from 2.6 to 15.1% and the accuracy was –22.1 to 16.6%. Application of the method was demonstrated by the analysis of mung bean (Vigna radiata) sprout extract, in which 14 of the 26 analytes were detected and quantified.