Molecular analysis of a cruciferin storage protein gene family of Brassica napus

We have isolated a five-member gene subfamily which encodes cruciferin, a legumin-like 12S storage protein of Brassica napus L., and have analyzed the structure and expression of the family members in developing embryos. Sequence analysis has shown that the coding regions of all five genes are highl...

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Bibliographic Details
Published inPlant molecular biology Vol. 19; no. 6; p. 1049
Main Authors Breen, J.P. (Indiana Univ., Bloomington, IN (USA). Dept. of Biology), Crouch, M.L
Format Journal Article
LanguageEnglish
Published Netherlands 01.09.1992
Subjects
Allergens
Antigens, Plant
Base Sequence
Brassica - genetics
BRASSICA NAPUS
DNA
EXPRESION GENICA
EXPRESSION DES GENES
GENE
GENE EXPRESSION
GENES
Molecular Sequence Data
Multigene Family
NUCLEOTIDE SEQUENCE
PLANT PROTEINS
Plant Proteins - genetics
PROTEINAS VEGETALES
PROTEINE VEGETALE
Restriction Mapping
SECUENCIA NUCLEICA
Seed Storage Proteins
Sequence Homology, Nucleic Acid
SEQUENCE NUCLEIQUE
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Summary:We have isolated a five-member gene subfamily which encodes cruciferin, a legumin-like 12S storage protein of Brassica napus L., and have analyzed the structure and expression of the family members in developing embryos. Sequence analysis has shown that the coding regions of all five genes are highly similar, with the two most divergent members of the family retaining 89% sequence identity. The analysis of this cruciferin gene family's expression indicates that the developmental pattern of expression of each gene is similar, and the steady-state mRNA levels of each gene are approximately equivalent to each other at all developmental stages.
Bibliography:F30
9204372
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00040536