Non-target effects of entomopathogenic nematodes on soil microbial community and nutrient cycling processes: A microcosm study

• Published in: Applied soil ecology : a section of Agriculture, ecosystems & environment Vol. 34; no. 2; pp. 250 - 257
• Main Authors: De Nardo, Elizabeth A.B., Grewal, Parwinder S., McCartney, David, Stinner, Benjamin R.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2006
• Subjects: Biological control; biological control agents; Entomopathogenic nematodes; Galleria mellonella; Microbial biomass; Microcosms; nitrogen; Nitrogen cycling; Non-target effects; nontarget organisms; Risks; soil ecology; Soil microbial activities; soil microorganisms; soil nutrient dynamics; Soil nutrient pools; soil nutrients; Soil respiration; Steinernema carpocapsae; trichlorfon; Risks; Biological control; Soil microbial activities; Entomopathogenic nematodes; Soil nutrient pools; Non-target effects; Soil respiration; Microbial biomass; Microcosms; Nitrogen cycling
• ISSN: 0929-1393; 1873-0272
• DOI: 10.1016/j.apsoil.2006.01.004

Under microcosm conditions, changes in the soil microbial biomass, respiration rates, and nitrogen pools as indicators of potential non-target effects of entomopathogenic nematodes on soil, were evaluated. Two tests were conducted using soil collected from the field with no history of entomopathogenic nematode application. Treatments consisted of applications of Steinernema carpocapsae All strain in the presence or absence of the wax moth Galleria mellonella larva as a target insect host, compared with the untreated control (soil only). In the second experiment an insecticide treatment (trichlorfon) was added. Microbial biomass (total nitrogen), and mineral nitrogen (NH 4-N, NO 3-N) were measured using standard methods up to 32 days and soil respiration up to 64 days in both experiments. No negative effect was detected in the soil microbial biomass, respiration and nitrogen pools after application of S. carpocapsae. However, a significant increase in ammonium was measured during almost the entire period of the test in the nematode plus larva treatment, not shown in the other treatments. This high level of ammonium in the nematode plus larva treatment showed that entomopathogenic nematodes can indirectly affect system-level processes in soil and adds evidence on the importance of indirect interactions affecting functions in soil food webs. The application of the insecticide trichlorfon significantly suppressed the microbial biomass and nitrification process.