A probiotic formulation containing Lactobacillus bulgaricus DWT1 inhibits tumor growth by activating pro-inflammatory responses in macrophages

• Published in: Journal of functional foods Vol. 56; pp. 232 - 245
• Main Authors: Guha, Dipanjan, Banerjee, Arka, Mukherjee, Raktim, Pradhan, Biswaranjan, Peneva, Maria, Aleksandrov, Georgi, Suklabaidya, Sujit, Senapati, Shantibhushan, Aich, Palok
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.05.2019
• Subjects: animal models; dietary supplements; functional foods; In vitro; In vivo; inflammation; Lactobacillus delbruckei sp. Bulgaricus DWT1; Lactobacillus delbrueckii subsp. bulgaricus; Macrophage polarization; macrophages; mice; neoplasms; Probiotic; probiotics; Streptococcus thermophilus; therapeutics; Tumor; Probiotic; In vivo; Tumor; Lactobacillus delbruckei sp. Bulgaricus DWT1; Macrophage polarization; In vitro
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2019.03.030

[Display omitted] •A probiotic formulation of Lactobacillus bulgaricus DTW1 was tested.•LDB polarized macrophages from M2 to M1 from in vitro to in vivo in mouse model.•LDB formulation was tested orally and intra-peritoneally.•LDB inhibited tumor growth in vivo in mouse model. Probiotics are functional foods and are becoming more evident with time that probiotics can have prophylactic and therapeutic roles. Current report revealed that an established probiotic cocktail of Lactobacillus delbruckei sp. bulgaricus DWT1 and Streptococcus thermophilus DWT4, could inhibit tumor growth. This probiotic cocktail is already marketed in various forms of food supplement. Present report reveals the transition of M2 to M1 like state of macrophage cells of mouse origin in vitro, ex vivo and in vivo following treatment with the established probiotic cocktail. Current study for the first time suggests the ability of a probiotic formulation to polarize macrophages from M2 to M1 state with potentials of developing newer intervention strategies for tumor growth. As a validation of our claim, we have established a novel and significant way of tumor inhibition in mouse model by the probiotic formulation that is capable of polarizing macrophages from M2 to M1.