Hybridization decreases native cutthroat trout reproductive fitness

• Published in: Molecular ecology Vol. 31; no. 16; pp. 4224 - 4241
• Main Authors: Rosenthal, William C., Fennell, John M., Mandeville, Elizabeth G., Burckhardt, Jason C., Walters, Annika W., Wagner, Catherine E.
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell Publishing Ltd 01.08.2022
• Subjects: conservation genetics; contemporary evolution; cutthroat trout; fish; Fitness; genomics/proteomics; Greenhouse effect; Hybridization; Hybrids; Juveniles; Mate selection; Natural selection; Offspring; Oncorhynchus clarkii bouvieri; Oncorhynchus mykiss; Populations; Reproductive fitness; Spawning; Trout
• ISSN: 0962-1083; 1365-294X
• DOI: 10.1111/mec.16578

Examining natural selection in wild populations is challenging, but crucial to understanding many ecological and evolutionary processes. Additionally, in hybridizing populations, natural selection may be an important determinant of the eventual outcome of hybridization. We characterized several components of relative fitness in hybridizing populations of Yellowstone cutthroat trout and rainbow trout in an effort to better understand the prolonged persistence of both parental species despite predictions of extirpation. Thousands of genomic loci enabled precise quantification of hybrid status in adult and subsequent juvenile generations; a subset of those data also identified parent–offspring relationships. We used linear models and simulations to assess the effects of ancestry on reproductive output and mate choice decisions. We found a relatively low number of late‐stage (F3+) hybrids and an excess of F2 juveniles relative to the adult generation in one location, which suggests the presence of hybrid breakdown decreasing the fitness of F2+ hybrids later in life. Assessments of reproductive output showed that Yellowstone cutthroat trout are more likely to successfully reproduce and produce slightly more offspring than their rainbow trout and hybrid counterparts. Mate choice appeared to be largely random, though we did find statistical support for slight female preference for males of similar ancestry. Together, these results show that native Yellowstone cutthroat trout are able to outperform rainbow trout in terms of reproduction and suggest that management action to exclude rainbow trout from spawning locations may bolster the now‐rare Yellowstone cutthroat trout.