Development of a novel signature derived from single cell RNA‐sequencing for preoperative prediction of lymph node metastasis in head and neck squamous cell carcinoma
Background Lymph node metastasis (LNM) is considered as an adverse prognostic indicator for cancer patients. Preoperative knowledge of LNM is valuable for pretreatment decision making. Here, we sought to develop and validate an LNM signature for preoperative prediction of LNM in patients with head a...
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Published in | Head & neck Vol. 44; no. 10; pp. 2171 - 2180 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken, USA
John Wiley & Sons, Inc
01.10.2022
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Background
Lymph node metastasis (LNM) is considered as an adverse prognostic indicator for cancer patients. Preoperative knowledge of LNM is valuable for pretreatment decision making. Here, we sought to develop and validate an LNM signature for preoperative prediction of LNM in patients with head and neck squamous cell carcinoma (HNSCC).
Methods
By studying single cell RNA‐sequencing data (scRNA‐seq), differentially expressed mRNA were selected and analyzed through univariate logistic regression and least absolute shrinkage and selection operator (LASSO) to identify an LNM signature. Multivariate logistic regression was utilized to establish an LNM nomogram incorporating LNM signature and T‐classification.
Results
The LNM signature was significantly associated with lymph node status and prognosis. The LNM signature and LNM nomogram displayed a robust predictive effect.
Conclusion
Our study reveals that LNM signature is a powerful biomarker for preoperative prediction of LNM in patients with HNSCC, which may be effective to realize individualized outcome prediction. |
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Bibliography: | Funding information Yibin Dai, Ziyu Wang, and Enshi Yan contributed equally to this article. Priority Academic Program Development of Jiangsu Higher Education Institutions, Grant/Award Number: 2018‐87; Nanjing Medical University Science and Technology Development Funding Project, Grant/Award Number: NMUB2020183; The Natural Scientific Foundation of the Jiangsu Higher Education Institutions of China, Grant/Award Number: 21KJB320011 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1043-3074 1097-0347 |
DOI: | 10.1002/hed.27126 |