Comparative Proteomic Analysis Reveals the Ascorbate Peroxidase-Mediated Plant Resistance to Verticillium dahliae in Gossypium barbadense

In previous research on the resistance of cotton to Verticillium wilt (VW), and were usually used as the susceptible and resistant cotton species, despite their different genetic backgrounds. Herein, we present data independent acquisition (DIA)-based comparative proteomic analysis of two cultivars...

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Published inFrontiers in plant science Vol. 13; p. 877146
Main Authors Lu, Tianxin, Zhu, Liping, Liang, Yuxuan, Wang, Fei, Cao, Aiping, Xie, Shuangquan, Chen, Xifeng, Shen, Haitao, Wang, Beini, Hu, Man, Li, Rong, Jin, Xiang, Li, Hongbin
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 19.05.2022
Subjects
ascorbate peroxidase
comparative proteomics
Gossypium barbadense
Plant Science
reactive oxygen species
Verticillium dahliae
Gossypium barbadense
Verticillium dahliae
ascorbate peroxidase
comparative proteomics
reactive oxygen species
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Summary:In previous research on the resistance of cotton to Verticillium wilt (VW), and were usually used as the susceptible and resistant cotton species, despite their different genetic backgrounds. Herein, we present data independent acquisition (DIA)-based comparative proteomic analysis of two cultivars differing in VW tolerance, susceptible XH7 and resistant XH21. A total of 4,118 proteins were identified, and 885 of them were differentially abundant proteins (DAPs). Eight co-expressed modules were identified through weighted gene co-expression network analysis. GO enrichment analysis of the module that significantly correlated with infection time revealed that oxidoreductase and peroxidase were the most significantly enriched GO terms. The last-step rate-limiting enzyme for ascorbate acid (AsA) biosynthesis was further uncovered in the significantly enriched GO terms of the 184 XH21-specific DAPs. Additionally, the expression of ascorbate peroxidase ( ) members showed quick accumulation after inoculation. Compared to XH7, XH21 contained consistently higher AsA contents and rapidly increased levels of expression, suggesting their potential importance for the resistance to . Silencing / in both XH7 and XH 21 resulted in a dramatic reduction in VW resistance. Our data indicate that APX-mediated oxidoreductive metabolism is important for VW resistance in cotton.
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Edited by: Shaojun Dai, Shanghai Normal University, China
Reviewed by: Shamim Hasan, University of Bonn, Germany; David D. Fang, Agricultural Research Service, United States Department of Agriculture, United States
These authors have contributed equally to this work
This article was submitted to Plant Proteomics and Protein Structural Biology, a section of the journal Frontiers in Plant Science
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2022.877146