A model for how Gβγ couples Gα to GPCR

• Published in: The Journal of general physiology Vol. 154; no. 5; p. 1
• Main Author: McIntire, William E
• Format: Journal Article
• Language: English
• Published: United States Rockefeller University Press 02.05.2022
• Subjects: Asparagine; Binding Sites; C-Terminus; Cell Membrane - metabolism; G protein-coupled receptors; Genomes; Humans; Hydrophobicity; Hypothesis; Intercellular Signaling; Isoforms; Molecular Physiology; Phenylalanine; Protein Isoforms - metabolism; Proteins; Receptors, G-Protein-Coupled - metabolism; Rhodopsin; Signal Transduction
• ISSN: 0022-1295; 1540-7748
• DOI: 10.1085/jgp.202112982

Representing ∼5% of the human genome, G-protein-coupled receptors (GPCRs) are a primary target for drug discovery; however, the molecular details of how they couple to heterotrimeric G protein subunits are incompletely understood. Here, I propose a hypothetical initial docking model for the encounter between GPCR and Gβγ that is defined by transient interactions between the cytosolic surface of the GPCR and the prenyl moiety and the tripeptide motif, asparagine-proline-phenylalanine (NPF), in the C-terminus of the Gγ subunit. Analysis of class A GPCRs reveals a conserved NPF binding site formed by the interaction of the TM1 and H8. Functional studies using differentially prenylated proteins and peptides further suggest that the intracellular hydrophobic core of the GPCR is a prenyl binding site. Upon binding TM1 and H8 of GPCRs, the propensity of the C-terminal region of Gγ to convert into an α helix allows it to extend into the hydrophobic core of the GPCR, facilitating the GPCR active state. Conservation of the NPF motif in Gγ isoforms and interacting residues in TM1 and H8 suggest that this is a general mechanism of GPCR-G protein signaling. Analysis of the rhodopsin dimer also suggests that Gγ-rhodopsin interactions may facilitate GPCR dimer transactivation.