Effects of dietary taurine, carnitine and cholesterol supplementation on growth performance and immunological status of Litopenaeus vannamei under cold exposure

• Published in: Aquaculture research Vol. 48; no. 3; pp. 1279 - 1290
• Main Authors: Zhou, Meng, Wu, Zaohe, Liang, Rishen, Gu, Na
• Format: Journal Article
• Language: English
• Published: Oxford Hindawi Limited 01.03.2017
• Subjects: carnitine; cholesterol; cold exposure; Decapoda; haemocyte; Litopenaeus vannamei; taurine
• ISSN: 1355-557X; 1365-2109
• DOI: 10.1111/are.12970

The aim of this study was to investigate effects of dietary feed additives on growth and immunological status of Litopenaeus vannamei under cold exposure. Four experimental diets with 0.2% taurine, 0.01% carnitine, 0.1% cholesterol supplementation and control were fed to L. vannamei (IBW 0.86 ± 0.03 g) for 30 days at 30 ± 1°C before water temperature was dropped to 16°C with a rate of −4°C h−1. Growth performance parameters including weight gain rate (WGR), feed conversion ratio (FCR) and survival rate before the cold exposure, as well as immunological parameters related to haemocytes including total haemocyte count (THC), percentages of hyaline cells (SC%), granular cells and semigranular cells (SC + GC%), non‐specific esterase (NSE) activity and apoptotic cell ratio before and after the cold exposure were determined. The results showed that: (1) WGR, FCE and survival rate were not significantly different among groups before the cold exposure. Most of immunological parameters did not differ either, except the apoptotic cell ratio of carnitine and cholesterol groups was significantly lower than that of control and taurine groups (P < 0.05). (2) After the cold exposure, THC was decreased by 48.1% (P < 0.05), 54.1% (P < 0.05), 30.1% and 2.2%; NSE activity was decreased by 31.7% (P < 0.05), 21.4%, 7.0% and 3.5% in control, taurine, carnitine and cholesterol respectively. (3) Conversely, ROS production was increased by 23.8% (P < 0.05), 15.1%, 7.2% and 13.0%; apoptotic cell ratio was increased by 10.4%, 12.8%, 6.4% and 7.2% in control, taurine, carnitine and cholesterol respectively. (4) An increase in HC%, decrease in SC + GC% in all groups was observed after cold exposure, and was significant in cholesterol group. This study showed that cholesterol, carnitine and taurine affected immunological status of L. vannamei under cold exposure to different degrees, possibly by modulating some immunological parameters, as discussed. Present study provides theoretical data for nutritional strategies to enhance cold resistance of shrimp.