Effect of α7n-Acetylcholine Receptor Activation and Antibodies to TNF-α on Mortality of Mice and Concentration of Proinflammatory Cytokines During Early Stage of Sepsis

• Published in: Bulletin of experimental biology and medicine Vol. 159; no. 6; pp. 740 - 742
• Main Authors: Zabrodskii, P. F., Gromov, M. S., Maslyakov, V. V.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2015
• Subjects: alpha7 Nicotinic Acetylcholine Receptor - agonists; Anabasine - pharmacology; Animals; Antibodies - pharmacology; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cytokines - blood; Disease Progression; Female; Inflammation Mediators - blood; Internal Medicine; Laboratory Medicine; Male; Mice; Pathology; Sepsis - blood; Sepsis - mortality; Sepsis - pathology; Time Factors; Tumor Necrosis Factor-alpha - antagonists & inhibitors; Tumor Necrosis Factor-alpha - immunology; antibodies to TNF-α; proinflammatory cytokines; cholinergic anti-inflammatory pathway; α7n-acetylcholine receptors; sepsis
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-015-3063-x

Experiments on random-bred albino mice showed that activation α7n-acetylcholine receptors with anabasine (0.5 LD50) and the use of antibodies to TNF-α (1 mg/kg) 2 h before sepsis modeling significantly reduces mortality of mice from experimental sepsis (intraperitoneal injection of E. coli ) due to a decrease in the blood concentration of proinflammatory cytokines TNF-α, IL-1β, and IL-6. After combined administration of anti-TNF-α antibodies and anabasine, an additive effect was observed.