Development and characterization of 11 microsatellite markers in the root-gall-forming weevil, Ceutorhynchus assimilis (Coleoptera: Curculionidae)

The host race of Ceutorhynchus assimilis Paykull, 1792 (Coleoptera: Curculionidae) that specifically develops on Lepidium draba Linné, 1753 (Brassicales: Brassicaceae), an invasive weed in North America, is being considered for use as a biocontrol agent. Above all, establishing the potential for gen...

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Bibliographic Details
Published inApplied entomology and zoology Vol. 51; no. 3; pp. 495 - 500
Main Authors Lesieur, V., Jeanneau, M., Martin, J. F., Bon, M. C.
Format Journal Article
LanguageEnglish
Published Tokyo Springer Japan 01.08.2016
Springer Nature B.V
Springer Verlag
Subjects
Agriculture
Applied Ecology
Biological control
Biomedical and Life Sciences
Ceutorhynchus assimilis
Coleoptera
Curculionidae
Entomology
Environmental Management
Gene flow
Gene loci
Genetic markers
Genomes
Life Sciences
Microsatellites
Pest control
Pests
Plant Pathology
Population genetics
Population studies
Technical Note
Zoology
NGS-enriched microsatellite library
Population genetics
Weed biological control
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Summary:The host race of Ceutorhynchus assimilis Paykull, 1792 (Coleoptera: Curculionidae) that specifically develops on Lepidium draba Linné, 1753 (Brassicales: Brassicaceae), an invasive weed in North America, is being considered for use as a biocontrol agent. Above all, establishing the potential for gene flow between this host race and the other entities within C. assimilis , some of which are pests, is crucial to resolving its taxonomic status, and will ultimately determine if a biocontrol program utilizing this agent can be pursued. Among the 92 microsatellite loci isolated from the C. assimilis genome using 454 pyrosequencing, 31 were retained based upon consistent amplification. Eleven loci exhibited polymorphism in populations sampled in France and Spain. In those populations, the number of alleles per locus ranged from 1 to 7, and the observed and expected heterozygosities ranged from 0.000 to 0.870 and 0.000 to 0.777, respectively. Principal coordinates analysis clearly separated the two populations. These polymorphic loci are the first microsatellites developed in C. assimilis , and represent the most informative markers available for fine- and large-scale population-genetic studies to date.
ISSN:0003-6862
1347-605X
DOI:10.1007/s13355-016-0414-7