Exploration of the virome of the European brown shrimp ( Crangon crangon )

• Published in: Journal of general virology Vol. 101; no. 6; pp. 651 - 666
• Main Authors: Van Eynde, Benigna, Christiaens, Olivier, Delbare, Daan, Shi, Chenyan, Vanhulle, Emiel, Yinda, Claude Kwe, Matthijnssens, Jelle, Smagghe, Guy
• Format: Journal Article
• Language: English
• Published: England 01.06.2020
• Subjects: virome; aquaculture; viruses; North Sea; Crangon crangon
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/jgv.0.001412

is economically a very important species. Recently, promising culture attempts have been made, but a major problem is the uncontrollable mortality during the grow-out phase. As of yet, the life cycle of is not closed in captivity so wild-caught individuals are used for further rearing. Therefore, it is important to investigate the virome of both in wild-caught animals as in cultured animals. In recent years, next-generation-sequencing (NGS) technologies have been very important in the unravelling of the virome of a wide range of environments and matrices, such as soil, sea, potable water, but also of a wide range of animal species. This will be the first report of a virome study in using NGS in combination with the NetoVIR protocol. The near complete genomes of 16 novel viruses were described, most of which were rather distantly related to unclassified viruses or viruses belonging to the , , , , , , , , . A difference in virome composition was observed between muscle and hepatopancreatic tissue, suggesting a distinct tissue tropism of several of these viruses. Some differences in the viral composition were noted between the cultured and wild shrimp, which could indicate that in sub-optimal aquaculture conditions some viruses become more abundant. This research showed that a plethora of unknown viruses is present in and that more research is needed to determine which virus is potentially dangerous for the culture of .