Enterobacter aerogenes ZDY01 Attenuates Choline-Induced Trimethylamine N -Oxide Levels by Remodeling Gut Microbiota in Mice

• Published in: Journal of microbiology and biotechnology Vol. 27; no. 8; pp. 1491 - 1499
• Main Authors: Qiu, Liang, Yang, Dong, Tao, Xueying, Yu, Jun, Xiong, Hua, Wei, Hua
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 28.08.2017
• Subjects: Animals; Asian Continental Ancestry Group; Biota; Cecum - chemistry; Enterobacter aerogenes - growth & development; Enterobacter aerogenes - isolation & purification; Gastrointestinal Microbiome; Gastrointestinal Tract - microbiology; Healthy Volunteers; Humans; Methylamines - metabolism; Mice; Oxidants - metabolism; Serum - chemistry; 생물학; trimethylamine; Enterobacter aerogenes; Trimethylamine N-oxide; gut microbiota
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1703.03039

Trimethylamine -oxide (TMAO), which is transformed from trimethylamine (TMA) through hepatic flavin-containing monooxygenases, can promote atherosclerosis. TMA is produced from dietary carnitine, phosphatidylcholine, and choline via the gut microbes. Previous works have shown that some small molecules, such as allicin, resveratrol, and 3,3-dimethyl-1-butanol, are used to reduce circulating TMAO levels. However, the use of bacteria as an effective therapy to reduce TMAO levels has not been reported. In the present study, 82 isolates were screened from healthy Chinese fecal samples on a basal salt medium supplemented with TMA as the sole carbon source. The isolates belonged to the family Enterobacteriaceae, particularly to genera , and . Serum TMAO and cecal TMA levels were significantly decreased in choline-fed mice treated with ZDY01 compared with those in choline-fed mice treated with phosphate-buffered saline. The proportions of Bacteroidales family S24-7 were significantly increased, whereas the proportions of Helicobacteraceae and Prevotellaceae were significantly decreased through the administration of ZDY01. Results indicated that the use of probiotics to act directly on the TMA in the gut might be an alternative approach to reduce serum TMAO levels and to prevent the development of atherosclerosis and "fish odor syndrome" through the effect of TMA on the gut microbiota.