Silicon-based ion channel sensor

In this paper we present a method to fabricate an aperture in a silicon wafer that can be used to suspend a freestanding lipid bilayer membrane. The design offers the feature of scalability of the aperture size into the submicron range. Lipid bilayer membranes formed across the aperture in the oxidi...

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Bibliographic Details
Published inSuperlattices and microstructures Vol. 34; no. 3; pp. 451 - 457
Main Authors Goryll, M, Wilk, S, Laws, G.M, Thornton, T, Goodnick, S, Saraniti, M, Tang, J, Eisenberg, R.S
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.09.2003
Subjects
Biosensors
Lipid bilayers
Silicon devices
Biosensors
Silicon devices
Lipid bilayers
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Summary:In this paper we present a method to fabricate an aperture in a silicon wafer that can be used to suspend a freestanding lipid bilayer membrane. The design offers the feature of scalability of the aperture size into the submicron range. Lipid bilayer membranes formed across the aperture in the oxidized silicon substrate show a gigaohm sealing resistance. The stability of these membranes allowed the insertion of a nanometer-sized ion channel protein (OmpF porin) and the measurement of voltage dependent gating that can be expected from a working porin ion channel.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:0749-6036
1096-3677
DOI:10.1016/j.spmi.2004.03.041