The effects of high calcium concentrations on renal ammoniagenesis by rat kidney slices

Deficiencies in acid excretion during hypercalcemia have been reported, and this defect has been ascribed to a deficiency in ammonia excretion. Because no changes in urine pH and urine flow occurred to explain decreased ammonia excretion, this suggested to us that decreased excretion was secondary t...

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Bibliographic Details
Published inNephron (2015) Vol. 17; no. 6; p. 474
Main Authors Vavatsi-Manos, O, Preuss, H G
Format Journal Article
LanguageEnglish
Published Switzerland 01.01.1976
Subjects
Ammonia - metabolism
Animals
Gluconeogenesis
Glutamates - metabolism
Glutamine - metabolism
Hypercalcemia - metabolism
In Vitro Techniques
Kidney - metabolism
Male
Oxygen Consumption
Rats
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Summary:Deficiencies in acid excretion during hypercalcemia have been reported, and this defect has been ascribed to a deficiency in ammonia excretion. Because no changes in urine pH and urine flow occurred to explain decreased ammonia excretion, this suggested to us that decreased excretion was secondary to decreased renal ammonia production. To test this hypothesis, we investigated the ability of kidney slices from rats to produce ammonia and glucose and to consume oxygen when incubated in varying concentrations of calcium. High medium concentrations of calcium (3 and 4 mM) decreased kidney slice ammonia-genesis from glutamine and glutamine and kidney slice oxygen consumption while not affecting gluconeogenesis. Based upon our findings, we propose that hypercalcemia decreases urine ammonia excretion by depressing renal ammonia production.
ISSN:1660-8151
DOI:10.1159/000180755