Synthesis of methotrexate polyglutamates by bone marrow cells from patients with leukemia and lymphoma

Bone marrow cells from children with lymphoblastic leukemia, myeloblastic leukemia and non-Hodgkin's lymphoma were incubated with tritium-labelled methotrexate in short-term cultures. Cells obtained both during disease activity as well as during remission synthesized large quantities of methotr...

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Bibliographic Details
Published inDevelopmental pharmacology and therapeutics Vol. 1; no. 1; p. 40
Main Authors Witte, A, Whitehead, V M, Rosenblatt, D S, Vuchich, M J
Format Journal Article
LanguageEnglish
Published Switzerland 1980
Subjects
Bone Marrow - metabolism
Cells, Cultured
Child
Dose-Response Relationship, Drug
Humans
Leukemia - metabolism
Lymphoma - metabolism
Methotrexate - analogs & derivatives
Methotrexate - biosynthesis
Methotrexate - metabolism
Peptide Biosynthesis
Polyglutamic Acid - analogs & derivatives
Polyglutamic Acid - biosynthesis
Time Factors
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Summary:Bone marrow cells from children with lymphoblastic leukemia, myeloblastic leukemia and non-Hodgkin's lymphoma were incubated with tritium-labelled methotrexate in short-term cultures. Cells obtained both during disease activity as well as during remission synthesized large quantities of methotrexate polyglutamates. Synthesis was time- and dose-dependent and occurred with concentrations of methotrexate regularly achieved during conventional treatment with this drug.
ISSN:0379-8305
DOI:10.1159/000455520