Two-dimensional thin-layer chromatography of Dns-amino acids on reversed-phase silica gel

Ready-for-use reversed-phase high-performance thin-layer chromatographic plates were used for the separation of Dns-amino acids. The development was performed by using methanol-2% acetic acid (75.25) or methanol-0.01 M Na2HPO4 (75:25). Reversed-phase plates were used also for adsorption chromatograp...

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Bibliographic Details
Published inJournal of chromatography Vol. 193; no. 3; p. 421
Main Authors Macek, K, Deyl, Z, Smrz, M
Format Journal Article
LanguageEnglish
Published Netherlands 30.05.1980
Subjects
Acetates
Adsorption
Amino Acids - analysis
Benzene
Chloroform
Chromatography, Thin Layer - methods
Dansyl Compounds - analysis
Gels
Heptanes
Indicators and Reagents
Methanol
Phosphates
Silicon Dioxide
Sodium
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Summary:Ready-for-use reversed-phase high-performance thin-layer chromatographic plates were used for the separation of Dns-amino acids. The development was performed by using methanol-2% acetic acid (75.25) or methanol-0.01 M Na2HPO4 (75:25). Reversed-phase plates were used also for adsorption chromatography using a non-aqueous solvent system such as benzene-chloroform-acetic acid (50:48:2) or n-heptane-ethyl acetate-acetic acid (65:33:2). Good separations were achieved by using both principles in the two-dimensional arrangement.
DOI:10.1016/S0021-9673(00)87742-0