Vitellibacter echinoideorum sp. nov., isolated from a sea urchin (Tripneustes gratilla)

• Published in: International journal of systematic and evolutionary microbiology Vol. 65; no. 7; pp. 2320 - 2325
• Main Authors: Lin, Shih-Yao, Hameed, Asif, Wen, Cheng-Zhe, Liu, You-Cheng, Hsu, Yi-Han, Shen, Fo-Ting, Lai, Wei-An, Young, Chiu-Chung
• Format: Journal Article
• Language: English
• Published: England 01.07.2015
• Subjects: Animals; Bacterial Typing Techniques; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Flavobacteriaceae - classification; Flavobacteriaceae - genetics; Flavobacteriaceae - isolation & purification; Molecular Sequence Data; Phosphatidylethanolamines - chemistry; Phylogeny; Pigmentation; RNA, Ribosomal, 16S - genetics; Sea Urchins - microbiology; Seafood - microbiology; Sequence Analysis, DNA; Taiwan; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; Taiwan
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.000258

A Gram-stain-negative, aerobic, rod-shaped, yellow-pigment-producing bacterium (designated strain CC-CZW007(T)) was isolated from seafood samples (sea urchins) at Penghu Island in Taiwan. Strain CC-CZW007(T) grew optimally at pH 7.0 and 30 °C in the presence of 3% (w/v) NaCl. The novel strain shared highest 16S rRNA gene sequence similarity to Vitellibacter vladivostokensis JCM 11732(T) (96.8%), Vitellibacter soesokkakensis KCTC 32536(T) (96.4%), Vitellibacter nionensis KCTC 32420(T) (95.8%) and Vitellibacter aestuarii JCM 15496(T) (95.6%) and lower sequence similarity to members of other genera. Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CZW007(T) with respect to other species of the genus Vitellibacter. The major fatty acids were iso-C15 : 0 and iso-C17 : 0 3-OH. The polar lipid profile was composed of major amounts of phosphatidylethanolamine, unidentified lipids and aminolipids; a moderate amount of aminophospholipid was also detected. The DNA G + C content was 34.7 mol%. The predominant quinone system was menaquinone (MK-6). On the basis of polyphasic taxonomic evidence presented here, strain CC-CZW007(T) is proposed to represent a novel species within the genus Vitellibacter, for which the name Vitellibacter echinoideorum sp. nov. is proposed. The type strain is CC-CZW007(T) ( = BCRC 80886(T) = JCM 30378(T)).