Somatic cell reference samples for calibration of milk somatic cell counting methods

Somatic cell count samples (SCCS) for use in calibration of milk somatic cell counting methods were prepared from raw bulk milk preserved with potassium dichromate. Somatic cells were separated by centrifugation, then appropriate cell dilutions were prepared in the dichromate-preserved skim milk. So...

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Bibliographic Details
Published inJournal of food protection Vol. 47; no. 9; pp. 694 - 696
Main Authors LINTNER, T. J, HEALD, C. W, EBERHART, R. J
Format Journal Article
LanguageEnglish
Published Des Moines, IA International Association of Milk, Food and Environmental Sanitarians 01.09.1984
Subjects
Biological and medical sciences
cell counting
conteo de celulas
Food industries
Fundamental and applied biological sciences. Psychology
lait
leche
milk
Milk and cheese industries. Ice creams
normalisation
normalizacion
numeration cellulaire
standardizing
Numeration
Somatic cell
Milk
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Summary:Somatic cell count samples (SCCS) for use in calibration of milk somatic cell counting methods were prepared from raw bulk milk preserved with potassium dichromate. Somatic cells were separated by centrifugation, then appropriate cell dilutions were prepared in the dichromate-preserved skim milk. Somatic cell counts from SCCS stored at 4°C were stable over a 23-wk period. No bacterial contamination was detected in these samples. In a collaborative study among eight laboratories, SCCS were not affected by usual conditions by shipping. The SCCS can be used as reference standards for the direct microscopic somatic cell count and the Fossomatic and Coulter Counter somatic cell counting methods.
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ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-47.9.694