Variations in the number of peptide-MHC class I complexes required to activate cytotoxic T cell responses

We determined equilibrium constants for the binding of 16 peptides (based on four T cell epitopes) to three MHC class I proteins (A2, Kb, and Ld) on intact cells and estimated the number of accessible peptide-binding sites on these cells. From these results, and the concentrations of peptides requir...

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Bibliographic Details
Published inThe Journal of immunology (1950) Vol. 154; no. 2; pp. 567 - 576
Main Authors Kageyama, S, Tsomides, TJ, Sykulev, Y, Eisen, HN
Format Journal Article
LanguageEnglish
Published United States Am Assoc Immnol 15.01.1995
Subjects
Amino Acid Sequence
Animals
Cell Line
Cytotoxicity Tests, Immunologic
H-2 Antigens - immunology
Histocompatibility Antigens Class I - immunology
HLA Antigens - immunology
Humans
Kinetics
Lymphocyte Activation - immunology
Mice
Molecular Sequence Data
Peptides - immunology
Protein Binding - immunology
T-Lymphocytes, Cytotoxic - immunology
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Summary:We determined equilibrium constants for the binding of 16 peptides (based on four T cell epitopes) to three MHC class I proteins (A2, Kb, and Ld) on intact cells and estimated the number of accessible peptide-binding sites on these cells. From these results, and the concentrations of peptides required to sensitize target cells for lysis by CD8+ CTL, we conclude that the critical number of peptide-MHC complexes required per target cell for the activation of CTL responses varies with different combinations of peptide-MHC complexes and CTL clones from several thousand complexes to fewer than ten per target cell.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.154.2.567