The Mixture of Anemarrhena asphodeloides and Coptis chinensis Attenuates High-Fat Diet-Induced Colitis in Mice

• Published in: The American journal of Chinese medicine (1979) Vol. 45; no. 5; p. 1033
• Main Authors: Lim, Su-Min, Choi, Hyun-Sik, Kim, Dong-Hyun
• Format: Journal Article
• Language: English
• Published: Singapore 2017
• Subjects: Adaptive Immunity - drug effects; Administration, Oral; Anemarrhena - chemistry; Animals; Cell Differentiation - drug effects; Colitis - drug therapy; Colitis - etiology; Coptis - chemistry; Diet, High-Fat - adverse effects; Drug Combinations; Drug Synergism; Gastrointestinal Microbiome - drug effects; Immunity, Innate - drug effects; Macrophage Activation - drug effects; Male; Mice, Inbred C57BL; NF-kappa B - metabolism; Peroxidase - metabolism; Phytotherapy; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; T-Lymphocytes, Regulatory - cytology; Colitis; The Mixture of Anemarrhena asphodeloides and Coptis chinensis; T Cell Differentiation; Macrophage Activation
• ISSN: 0192-415X
• DOI: 10.1142/S0192415X17500550

Anemarrhena asphodeloides (AA, family Liliaceae) inhibits macrophage activation by inhibiting IRAK1 phosphorylation and helper T (Th)17 differentiation. Coptis chinensis (CC, family Ranunculaceae), which inhibits macrophage activation by inhibiting the binding of lipopolysaccharide (LPS) on toll-like receptor 4 and inducing regulatory T (Treg) cell differentiation. The mixture of AA and CC (AC-mix) synergistically attenuates 2,4,6-trinitrobenzenesulfonic acid or dextran sulfate sodium-induced colitis in mice by inhibiting NF-[Formula: see text]B activation and regulating Th17/Treg balance. In the present study, we examined the effect of AC-mix on high-fat diet (HFD)-induced colitis in mice, which induced NF-[Formula: see text]B activation and disturbed Th17/Treg balance. Long-term feeding of HFD in mice caused colitis, including increased macroscopic score and myeloperoxidase activity. Oral administration of AC-mix (20[Formula: see text]mg/kg) suppressed HFD-induced myeloperoxidase activity by 68% ([Formula: see text]). Furthermore, treatment with the AC-mix (20[Formula: see text]mg/kg) inhibited HFD-induced activation of NF-[Formula: see text]B and expression of cyclooxygenase-2, inducible NO synthase, interleukin (IL)-17, and tumor necrosis factor-alpha but increased HFD- suppressed expression of IL-10. AC-mix suppressed HFD-induced differentiation into Th17 cells by 46% ([Formula: see text]) and increased HFD-induced differentiation into regulatory T cells 2.2-fold ([Formula: see text]). AC-mix also suppressed the HFD-induced Proteobacteria/Bacteroidetes ratio on the gut microbiota by 48% ([Formula: see text]). These findings suggest that AC-mix can ameliorate HFD-induced colitis by regulating innate and adaptive immunities and correcting the disturbance of gut microbiota.