Improving axial resolution for holographic tracking of colloids and bacteria over a wide depth of field by optimizing different factors

Improving the axial resolution for multiparticle three-dimensional (3D) holographic tracking is crucial but challenging. Here we study the impacts of incident light power, uniformity of the illumination as well as image pixel size on the axial tracking resolution for digital holographic microscopy (...

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Bibliographic Details
Published inOptics express Vol. 26; no. 8; pp. 9920 - 9930
Main Authors Huang, Gui, Tian, Wenzhang, Qi, Meng, Gong, Xiangjun, Zhang, Guangzhao
Format Journal Article
LanguageEnglish
Published United States 16.04.2018
Subjects
Algorithms
Bacteria
Colloids
Holography - methods
Image Processing, Computer-Assisted
Imaging, Three-Dimensional - methods
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Summary:Improving the axial resolution for multiparticle three-dimensional (3D) holographic tracking is crucial but challenging. Here we study the impacts of incident light power, uniformity of the illumination as well as image pixel size on the axial tracking resolution for digital holographic microscopy (DHM). We demonstrate that the resolution highly depends on the image pixel size and the uniformity of the illumination. A 3D localization algorithm based on local-intensity-maxima searching and a Gaussian fit to the integrated intensity of the reconstructed lateral images along the axial direction proves a robust strategy to enhance the axial resolution for colloids and bacteria within a wide depth of field over several tens of micrometers.
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ISSN:1094-4087
1094-4087
DOI:10.1364/OE.26.009920