Identification of crustacea allergens by crossed radioimmunoelectrophoresis

Crossed immunoelectrophoresis (CIE) detected 18 precipitating antigens in extracts of shrimp. Of these antigens, crossed-line immunoelectrophoresis (CLIE) of shrimp extract demonstrated that 5 cross-reacted with crayfish, 3 with lobster and 1 with crab extract. Allergens present in the shrimp CIE pl...

Full description

Saved in:
Bibliographic Details
Published inInternational archives of allergy and applied immunology Vol. 77; no. 1-2; p. 192
Main Authors Lehrer, S B, McCants, M L, Salvaggio, J E
Format Journal Article
LanguageEnglish
Published Switzerland 01.01.1985
Subjects
Allergens - immunology
Animals
Cross Reactions
Crustacea - immunology
Food Hypersensitivity - immunology
Humans
Immunoelectrophoresis, Two-Dimensional
Radioallergosorbent Test
Shellfish - adverse effects
Online AccessGet more information

Cover

More Information
Summary:Crossed immunoelectrophoresis (CIE) detected 18 precipitating antigens in extracts of shrimp. Of these antigens, crossed-line immunoelectrophoresis (CLIE) of shrimp extract demonstrated that 5 cross-reacted with crayfish, 3 with lobster and 1 with crab extract. Allergens present in the shrimp CIE plates were identified by crossed radioimmunoelectrophoresis (CRIE) using sera from 6 study subjects who were skin-test and RAST positive to shrimp extract. Of the 7 allergens detected, 3 (precipitins 1, 3 and 6) reacted with most of the 6 sera tested from shrimp-sensitive subjects. Precipitins 1 and 6 appear to be common crustacea allergens (present in shrimp, crayfish, lobster and crab) whereas precipitin 3 may be a specific allergen since it is present only in shrimp.
ISSN:0020-5915
DOI:10.1159/000233783