MicroRNA-92 promotes gastric cancer cell proliferation and invasion through targeting FXR

• Published in: Tumor biology Vol. 35; no. 11; pp. 11013 - 11019
• Main Authors: Duan, Jian-Hua, Fang, Long
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01.11.2014
• Subjects: Adult; Apoptosis; Blotting, Western; Cell adhesion & migration; Cell Cycle; Cell growth; Cell Movement; Cell Proliferation; Female; Gastric cancer; Gene Expression Regulation, Neoplastic; Humans; Male; MicroRNAs; MicroRNAs - genetics; Middle Aged; Neoplasm Invasiveness; Neoplasm Staging; Prognosis; Protein expression; Real-Time Polymerase Chain Reaction; Receptors, Cytoplasmic and Nuclear - antagonists & inhibitors; Receptors, Cytoplasmic and Nuclear - genetics; Receptors, Cytoplasmic and Nuclear - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Small Interfering - genetics; Stomach - metabolism; Stomach - pathology; Stomach Neoplasms - genetics; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Tumor Cells, Cultured
• ISSN: 1010-4283; 1423-0380
• DOI: 10.1007/s13277-014-2342-x

MicroRNAs (miRNAs), a class of small noncoding RNAs, play critical roles in human carcinogenesis through downregulation of various target genes. In the present study, we found that miR-92 is upregulated in gastric cancer tissues compared with adjacent normal tissues. Interestingly, miR-92 expression is significantly associated with clinical characteristics of patients. Gain or loss-of-function in vitro experiments further show that miR-92 mimics significantly promoted, while its antisense oligos inhibited gastric cancer cell proliferation and invasion. Moreover, luciferase reporter assays and western blot indicated that farnesoid X receptor (FXR), is a direct target of miR-92. Therefore, our data suggest that upregulation of miR-92 may represent an important mechanism for the development of gastric cancer.