Endothelial nitric oxide synthase/soluble guanylate cyclase system in human nasal polyps

• Published in: European archives of oto-rhino-laryngology Vol. 262; no. 6; pp. 512 - 516
• Main Authors: ESCOBAR, Paloma, MARQUEZ, Francisco J, CARRASCO, Carolina, ALONSO-ORGAZ, Sergio, FORTES, José, CENJOR, Carlos, LOPEZ-FARRE, Antonio
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.06.2005
• Subjects: Adult; Aged; Biological and medical sciences; Blotting, Western; Case-Control Studies; Female; Guanylate Cyclase - immunology; Guanylate Cyclase - metabolism; Humans; Immunohistochemistry; Male; Medical sciences; Middle Aged; Nasal Mucosa - enzymology; Nasal Polyps - enzymology; Nitric Oxide Synthase Type III - immunology; Nitric Oxide Synthase Type III - metabolism; Otorhinolaryngology. Stomatology; Human; Nose disease; Nasal polyp; Enzyme; Mucosa; Phosphorus-oxygen lyases; Lyases; Guanylate cyclase; Nitric-oxide synthase; Nasal mucosa; Endothelium; Nitric oxide; Cyclic GMP; ENT disease; Oxidoreductases; Polyps
• ISSN: 0937-4477; 1434-4726
• DOI: 10.1007/s00405-004-0857-2

The aim of our study was to analyze the level of expression of the endothelial nitric oxide synthase (eNOS)/soluble guanylate cyclase (sGC) system in nasal polyps and control nasal mucosae. The study was performed in polyps from 15 patients and nasal mucosae from 11 subjects operated on the nasal septum (control group). The expression of endothelial nitric oxide synthase (eNOS) and soluble guanylate cyclase (sGC) was determined in nasal mucosae. Western blot analysis demonstrated that eNOS protein was overexpressed in the nasal polyps with respect to control nasal mucosae. Immunohistochemistry also demonstrated that the vascular endothelium of nasal polyps contained higher amounts of eNOS protein than control nasal mucosae. Moreover, the beta(1) subunit of sGC was also overexpressed in the nasal polyps, which was associated with an increased content of cyclic GMP in the nasal polyps with respect to nasal control mucosae. In human nasal polyposis, there is an overexpression of the eNOS/sGC system. Further studies are needed to assess whether this overexpression is involved in the genesis of nasal polyposis.