The first encapsulation of hydroxynitrile lyase from Hevea brasiliensis in a sol–gel matrix
A straightforward process for the encapsulation of HbHNL under low methanol conditions has been developed. By adding a sol, prepared by hydrolysis of TMOS/MTMS at pH 2.8 with continuous removal of methanol, to a stirred solution of the enzyme in a buffer at pH 6.5, at least 65% of the activity of th...
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Published in | Tetrahedron Vol. 60; no. 46; pp. 10419 - 10425 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ltd
08.11.2004
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | A straightforward process for the encapsulation of
HbHNL under low methanol conditions has been developed. By adding a sol, prepared by hydrolysis of TMOS/MTMS at pH 2.8 with continuous removal of methanol, to a stirred solution of the enzyme in a buffer at pH 6.5, at least 65% of the activity of the free enzyme could be recovered after the encapsulation. The aquagels were successfully used in the synthesis of (
S)-cyanohydrins.
Graphical Abstract |
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ISSN: | 0040-4020 1464-5416 |
DOI: | 10.1016/j.tet.2004.06.135 |