The first encapsulation of hydroxynitrile lyase from Hevea brasiliensis in a sol–gel matrix

A straightforward process for the encapsulation of HbHNL under low methanol conditions has been developed. By adding a sol, prepared by hydrolysis of TMOS/MTMS at pH 2.8 with continuous removal of methanol, to a stirred solution of the enzyme in a buffer at pH 6.5, at least 65% of the activity of th...

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Bibliographic Details
Published inTetrahedron Vol. 60; no. 46; pp. 10419 - 10425
Main Authors Veum, Lars, Hanefeld, Ulf, Pierre, Alain
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 08.11.2004
Elsevier
Subjects
Asymmetric catalysis
Catalysis
Chemical Sciences
Cyanohydrins
Hydroxynitrile lyase
Immobilisation
Oxynitrilase
Sol–gel process
Oxynitrilase
Hydroxynitrile lyase
Sol–gel process
Asymmetric catalysis
Cyanohydrins
Immobilisation
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Summary:A straightforward process for the encapsulation of HbHNL under low methanol conditions has been developed. By adding a sol, prepared by hydrolysis of TMOS/MTMS at pH 2.8 with continuous removal of methanol, to a stirred solution of the enzyme in a buffer at pH 6.5, at least 65% of the activity of the free enzyme could be recovered after the encapsulation. The aquagels were successfully used in the synthesis of ( S)-cyanohydrins. Graphical Abstract
ISSN:0040-4020
1464-5416
DOI:10.1016/j.tet.2004.06.135