The Ala55Val and -866G>A polymorphisms of the UCP2 gene could be biomarkers for weight loss in patients who had Roux-en-Y gastric bypass

• Published in: Nutrition (Burbank, Los Angeles County, Calif.) Vol. 33; pp. 326 - 330
• Main Authors: Nicoletti, Carolina F., de Oliveira, Ana Paula R.P., Brochado, Maria José F., Pinhel, Marcela A.S., de Oliveira, Bruno A.P., Marchini, Julio S., dos Santos, José E., Salgado Jr, Wilson, Cury, Nathalia Moreno, de Araújo, Luiza Ferreira, Silva, Wilson A., Nonino, Carla B.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2017; Elsevier Limited
• Subjects: Adult; Alleles; Amino Acid Substitution; Anatomy & physiology; Bariatric surgery; Biomarker; Biomarkers; Body Composition; Body Mass Index; Brazil; Combined Modality Therapy; Ethics; Expenditures; Female; Gastric Bypass; Gastrointestinal surgery; Gene Frequency; Genes; Genetic Association Studies; Genotype & phenotype; Genotypes; Humans; Longitudinal Studies; Male; Medical records; Metabolism; Middle Aged; Mortality; Mutation, Missense; Obesity; Obesity, Morbid - genetics; Obesity, Morbid - metabolism; Obesity, Morbid - surgery; Obesity, Morbid - therapy; Polymorphism, Single Nucleotide; Studies; UCP2 polymorphism; Uncoupling Protein 2 - genetics; Uncoupling Protein 2 - metabolism; Weight control; Weight Loss; Obesity; Biomarker; UCP2 polymorphism; Bariatric surgery; Weight loss
• ISSN: 0899-9007; 1873-1244
• DOI: 10.1016/j.nut.2016.07.020

The aim of this study was to investigate whether the Ala55Val and -866G>A polymorphisms of the UCP2 gene are related to weight loss and changes in body composition after bariatric surgery performed by Roux-en-Y gastric bypass (RYGB). This longitudinal study enrolled obese patients submitted to RYGB. Data regarding weight (kg), body mass index (kg/m2), fat-free mass (FFM; kg), fat mass (kg), weight loss (kg and %), and percent excess weight loss were collected from both preoperative and 1-y postoperative medical records. Polymorphisms were genotyped by allelic discrimination using real-time polymerase chain reaction and TaqMan-predesigned single nucleotide polymorphism Genotyping Assay kits (Applied Biosystems, Foster City, CA, USA). The t test was used to compare variables between genotypes of each polymorphism to analyze the dominant and recessive models. Linear regression models were used to adjust the effects of initial weight, age, and sex on the variation of weight and body composition (P < 0.05). We analyzed 150 severely obese individuals (age 47.2 ± 10.5 y; 80% women). Genotype analysis showed a greater prevalence of heterozygous GA (41.3%) for -866G>A polymorphism and CT (39.3%) for Ala55Val polymorphism. Individuals who carried the T (CT+TT) and A (GA+AA) mutated alleles for Ala55Val and -866G>A, respectively, showed a higher weight and FFM loss. The mutated alleles T for Ala55Val and A for -866G>A polymorphism could be biomarkers of weight loss 1 y after RYGB. •Genotype analysis showed a greater prevalence of heterozygous for uncoupling protein (UCP)2 polymorphisms.•Individuals who carried mutated alleles for UCP2 polymorphisms showed a greater loss of weight and fat-free mass.•The mutated alleles for UCP2 polymorphism could be biomarkers of weight loss 1 y after Roux-en-Y gastric bypass.