Comparison of Immunoassay and HPLC for Analyzing Fluridone Concentrations: New Applications for Immunoassay Techniques

• Published in: Lake and reservoir management Vol. 18; no. 1; pp. 75 - 80
• Main Authors: Netherland, Michael D., Honnell, David R., Staddon, Alicia G., Getsinger, Kurt D.
• Format: Journal Article
• Language: English
• Published: Taylor & Francis Group 01.03.2002
• Subjects: aquatic herbicide; ELISA; herbicide residue; HPLC; immunoassay; Sonar
• ISSN: 1040-2381; 2151-5530
• DOI: 10.1080/07438140209353931

High performance liquid chromatography (HPLC) and an enzyme-linked immunosorbent assay (ELISA) technique were used to analyze concentrations of the aquatic herbicide fluridone (l-methyl-3 phenyl-5-[-3 (trifluromethyl) phenyl]-4 (IH) pyridinone) in 488 surface water samples collected from two lakes in Michigan treated in 1997, 2 Michigan lakes treated in 1998, 1 lake in Florida treated in 1996, and a series of research ponds treated in Florida in 1997. Samples were collected following application of fluridone associated with operational treatment programs that targeted the exotic submersed plants Myriophyllum spicatum L. (Michigan) and Hydrilla verticillata (L.f) Royle (Florida). ELISA and HPLC results compared well (r 2 = 0.84 to 0.98) across a broad range of initial fluridone treatment rates (5 to 150 μg L −1 ). The potential use of ELISA to monitor fluridone residues in the water in near real-time, and to use this residue data for lake specific herbicide treatment recommendations represents a unique use of ELISA technology.