Differentiation of normal and leukemic cells by 2D light scattering label-free static cytometry

Two-dimensional (2D) light scattering patterns of single microspheres, normal granulocytes and leukemic cells are obtained by label-free static cytometry. Statistical results of experimental 2D light scattering patterns obtained from standard microspheres with a mean diameter of 4.19 μm agree well w...

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Bibliographic Details
Published inOptics express Vol. 24; no. 19; pp. 21700 - 21707
Main Authors Xie, Linyan, Liu, Qiao, Shao, Changshun, Su, Xuantao
Format Journal Article
LanguageEnglish
Published United States 19.09.2016
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Summary:Two-dimensional (2D) light scattering patterns of single microspheres, normal granulocytes and leukemic cells are obtained by label-free static cytometry. Statistical results of experimental 2D light scattering patterns obtained from standard microspheres with a mean diameter of 4.19 μm agree well with theoretical simulations. High accuracy rates (greater than 92%) for label-free differentiation of normal granulocytes and leukemic cells, both the acute and chronic leukemic cells, are achieved by analyzing the 2D light scattering patterns. Our label-free static cytometry is promising for leukemia screening in clinics.
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ISSN:1094-4087
1094-4087
DOI:10.1364/OE.24.021700