Standardization of PCR technique for detecting Listeria monocytogenes in chicken, beef and pork

The aim of this study was to standardize the PCR technique for detecting Listeria monocytogenes (Lm) in chicken, beef and pork. The sensitivity and specicity of PCR and the level of concordance with the microbiological method were evaluated. PCR was standardized using a total of 60 samples of chicke...

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Published inEcosistemas y Recursos Agropecuarios Vol. 5; no. 13; pp. 25 - 34
Main Authors De la Rosa Zariñana, Asael Edem, Crosby-Galván, María M., Ramírez-Guzmán, Martha E., Hernández-Sánchez, David, Mata-Espinoza, Miguel A.
Format Journal Article
LanguageEnglish
Published Universidad Juárez Autónoma de Tabasco 01.01.2018
Subjects
Bayes' Theorem
Kappa index
Listeria monocytogenes
meat
PCR
sensitivity
validation
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Summary:The aim of this study was to standardize the PCR technique for detecting Listeria monocytogenes (Lm) in chicken, beef and pork. The sensitivity and specicity of PCR and the level of concordance with the microbiological method were evaluated. PCR was standardized using a total of 60 samples of chicken, beef and pork (20 samples per meat type.) Sensitivity and specicity were calculated using the 2 x 2 table and the level of accordance by the Kappa index. The minimum detectable DNA concentration was 3.0 ng L???1. The PCR showed 100% sensitivity andspecicity, and 80, 91 and 100% concordance between the methods was obtained for detecting Lm in chicken, beef and pork samples respectively, with 89.4% similarity between the methods for the three types of meat.
ISSN:2007-9028
2007-901X
DOI:10.19136/era.a5n13.1428