Measurement of vitamin K in human liver by gradient elution high-performance liquid chromatography using platinum-black catalyst reduction and fluorimetric detection

A sensitive and precise method for measuring endogenous phylloquinone (K 1) and menaquinone (MK- n) in human liver was developed, based on gradient elution high-performance liquid chromatography using platinum-black catalyst reduction and fluorimetric detection. Subnanogram levels of vitamin K compo...

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Bibliographic Details
Published inJournal of chromatography Vol. 489; no. 2; pp. 291 - 301
Main Authors Usui, Yuji, Nishimura, Naohiko, Kobayashi, Nobuaki, Okanoue, Toyotake, Kimoto, Michio, Ozawa, Kazue
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 1989
Subjects
Catalysis
Chromatography, High Pressure Liquid
Humans
Liver - analysis
Mitochondria, Liver - analysis
Oxidation-Reduction
Platinum
Spectrometry, Fluorescence
Vitamin K - analysis
Vitamin K 1 - analysis
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Summary:A sensitive and precise method for measuring endogenous phylloquinone (K 1) and menaquinone (MK- n) in human liver was developed, based on gradient elution high-performance liquid chromatography using platinum-black catalyst reduction and fluorimetric detection. Subnanogram levels of vitamin K compounds in 1 g of liver specimen were detectable. We measured vitamin K concentrations in 38 human resected livers. K 1 and MK-4 to MK-13 were detected. The concentrations of MK-10 to MK-12 in livers with chronic hepatitis ( n=10) and cirrhosis ( n=22) were significantly lower than in normal livers ( n=6). It is suggested that the decreased concentrations indicate functional damage of the hepatocytes.
ISSN:0378-4347
DOI:10.1016/S0378-4347(00)82907-2