L-Malic acid biosensor for field-based evaluation of apple, potato and tomato horticultural produce

• Published in: Analyst (London) Vol. 127; no. 1; pp. 104 - 108
• Main Authors: ARIF, Mobeen, SETFORD, Steven J, BURTON, Kerry S, TOTHILL, Ibtisam E
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 01.01.2002
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Biosensing Techniques; Biosensors; Biotechnology; Economic plant physiology; Fructification, ripening. Postharvest physiology; Fruit - chemistry; Fundamental and applied biological sciences. Psychology; Growth and development; Lycopersicon esculentum; Malates - analysis; Malus; Methods. Procedures. Technologies; Solanum tuberosum; Various methods and equipments; Performance evaluation; Enzyme; Electrode poisoning; ); Enzyme electrode; Electrochemical detector; Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP; Ion selective electrode; Hydroxyacid; Vegetable crop; Agronomy; Biosensor; Dicarboxylic acid; Malic acid; Oxidoreductases; Immobilized enzyme
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/b106400m

A screen-printed three-electrode amperometric biosensor incorporating malic enzyme for the measurement of L-malic acid in apple, potato and tomato horticultural samples has been developed. The working electrode contained 0.38 mU of immobilised enzyme and was fabricated using rhodinised carbon to facilitate NADPH oxidation at an operating potential of +300 mV vs. Ag/AgCl compared with > +600 mV for bare carbon. The linear range of the sensor was 0.028-0.7 mM L-malic acid with relative standard deviations of 3.3-13.3%. When testing with real apple, potato and tomato samples, the sensor accuracy was within 13.7% of a standard commercially available photometric test kit. The sensor approach is cheap, simple to perform and rapid (6 min), requiring only buffer-electrolyte and a small sample volume.