Segmentation and 3D Reconstruction of Microbial Biofilms

Capability of microbial biofilms to form biofilms is really important in biotechnology industries, environmental applications and medical applications. Therefore, the study of the structure and development of these organisms is essential. In this paper we develop and implement an algorithm, based on...

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Bibliographic Details
Published inRevista IEEE América Latina Vol. 11; no. 1; pp. 324 - 328
Main Authors Bouchet, A., Pastore, J. I., Di Meglio, L., Robuschi, L., Ballarin, V.
Format Journal Article
LanguageEnglish
Published Los Alamitos IEEE 01.02.2013
The Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subjects
3D Reconstruction
Algorithms
Biofilms
Image contrast
Image segmentation
In vivo
Mathematical Morphology
Microorganisms
Morphology
Reconstruction
Segmentation
Three dimensional
Three-dimensional displays
Visualization
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Summary:Capability of microbial biofilms to form biofilms is really important in biotechnology industries, environmental applications and medical applications. Therefore, the study of the structure and development of these organisms is essential. In this paper we develop and implement an algorithm, based on Mathematical Morphology, to analyze the structure and growing of microbial biofilms. This algorithm allows segmentation of a stack of images acquired from in vivo observation of microorganisms, using an inverted phase contrast microscope. Finally, a 3D reconstruction for an efficient visualization of the structure and topography of biofilms is done.
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ISSN:1548-0992
1548-0992
DOI:10.1109/TLA.2013.6502824