SOCS1 acts as a ferroptosis driver to inhibit the progression and chemotherapy resistance of triple-negative breast cancer

• Published in: Carcinogenesis (New York) Vol. 44; no. 8-9; pp. 708 - 715
• Main Authors: Wang, Yiding, Pang, Xiaoling, Liu, Yuexin, Mu, Guiling, Wang, Qian
• Format: Journal Article
• Language: English
• Published: UK Oxford University Press 02.12.2023
• Subjects: Animals; Cell Line, Tumor; Cell Proliferation - genetics; Cisplatin - pharmacology; Disease Models, Animal; Ferroptosis - genetics; Humans; Suppressor of Cytokine Signaling 1 Protein - genetics; Suppressor of Cytokine Signaling 1 Protein - metabolism; Suppressor of Cytokine Signaling Proteins - metabolism; Triple Negative Breast Neoplasms - drug therapy; Triple Negative Breast Neoplasms - genetics; Triple Negative Breast Neoplasms - metabolism
• ISSN: 0143-3334; 1460-2180
• DOI: 10.1093/carcin/bgad060

Abstract Objectives Ferroptosis is involved in many types of cancers, including triple-negative breast cancer (TNBC). Suppressor of cytokine signaling 1 (SOCS1) has recently been implicated as a regulator of ferroptosis. We aim to explore whether targeting SOCS1 is a potential therapeutic strategy for TNBC therapy. Methods Stable cell lines were constructed using lentivirus transfection. Cell viability was determined using CCK-8 and cell colony formation assays, respectively. Assays including lactate dehydrogenase release, lipid peroxidation and malondialdehyde assays were conducted to evaluate ferroptosis. Real-time quantitative polymerase chain reaction and western blotting were performed to evaluate mRNA and protein expression, respectively. A xenograft animal model was established by subcutaneous injection of cells into the flank. Results Our results showed that SOCS1 overexpression inhibited cell proliferation and induced ferroptosis in TNBC cells, while SOCS1 knockdown promoted cell proliferation and reduced ferroptosis. We also found that SOCS1 regulated ferroptosis by modulating GPX4 expression. Furthermore, SOCS1 regulated cisplatin resistance in TNBC cells by promoting ferroptosis. Our in vivo data suggested that SOCS1 regulated tumor growth and cisplatin resistance in vivo. Conclusions SOCS1 inhibits the progression and chemotherapy resistance of TNBC by regulating GPX4 expression. Graphical Abstract Graphical Abstract