A novel fluorescent detection for PDGF-BB based on dsDNA-templated copper nanoparticles

A novel method for the detection of PDGF-BB has been developed using double-strand DNA-copper nanoparticles （dsDNA-CuNPs） as fluorescent markers. This assay relies on the premise that the aptamer- based probe undergoes a conformational change upon binding with target protein, and subsequently trigge...

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Bibliographic Details
Published inChinese chemical letters Vol. 25; no. 1; pp. 9 - 14
Main Authors Yang, Xiao-Hai, Sun, Shan, Liu, Pei, Wang, Ke-Min, Wang, Qing, Liu, Jian-Bo, Huang, Jin, He, Lei-Liang
Format Journal Article
LanguageEnglish
Published Elsevier B.V 2014
Subjects
Copper nanoparticles
PDGF-BB
Polymerization
双链DNA
构象变化
检测灵敏度
模板化
荧光标记
荧光检测
选择性检测
铜纳米粒子
Polymerization
Copper nanoparticles
PDGF-BB
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Summary:A novel method for the detection of PDGF-BB has been developed using double-strand DNA-copper nanoparticles （dsDNA-CuNPs） as fluorescent markers. This assay relies on the premise that the aptamer- based probe undergoes a conformational change upon binding with target protein, and subsequently triggers polymerization reaction to generate dsDNA. Then, the resultant dsDNA can be used as a template for the formation of CuNPs with high fluorescence. Under the optimized conditions, the proposed assay allowed sensitive and selective detection of PDGF-BB with a detection limit of 4 nmol/L. This possibly makes it an attractive platform for the detection of a variety of biomolecules whose aptamers undergo similar conformational change.
Bibliography:11-2710/O6
Copper nanoparticles PDGF-BB Polymerization
A novel method for the detection of PDGF-BB has been developed using double-strand DNA-copper nanoparticles （dsDNA-CuNPs） as fluorescent markers. This assay relies on the premise that the aptamer- based probe undergoes a conformational change upon binding with target protein, and subsequently triggers polymerization reaction to generate dsDNA. Then, the resultant dsDNA can be used as a template for the formation of CuNPs with high fluorescence. Under the optimized conditions, the proposed assay allowed sensitive and selective detection of PDGF-BB with a detection limit of 4 nmol/L. This possibly makes it an attractive platform for the detection of a variety of biomolecules whose aptamers undergo similar conformational change.
ISSN:1001-8417
1878-5964
DOI:10.1016/j.cclet.2013.10.032