Characterization of a Procine Amelogenin Preparation, EMADOGAIN, a Biological Treatment for Periodontal Disease

EMDOGAIN is derived from porcine developing enamel matrix and has been shown to facilitate regeneration of the periodontium, although its mechanism of action is unknown. The aim of the present study was to identify enamel matrix proteins and proteolytic enzymes present in EMDOGAIN and compare them w...

Full description

Saved in:
Bibliographic Details
Published inConnective tissue research Vol. 43; no. 2-3; pp. 472 - 476
Main Authors Maycock, J., Wood, S. R., Brookes, S. J., Shore, R. C., Robinson, C., Kirkham, J.
Format Journal Article
LanguageEnglish
Published Informa UK Ltd 2002
Taylor & Francis
Subjects
Amelogenin
Emdogain
Periodontal Regeneration
Online AccessGet full text

Cover

More Information
Summary:EMDOGAIN is derived from porcine developing enamel matrix and has been shown to facilitate regeneration of the periodontium, although its mechanism of action is unknown. The aim of the present study was to identify enamel matrix proteins and proteolytic enzymes present in EMDOGAIN and compare them with those extracted from developing porcine enamel itself. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting, and zymography were used to identify the proteins present and to determine their enzyme activity. The results showed that developing enamel contained amelogenins, albumin, amelin, and enamelin. EMDOGAIN, however, contained only amelogenins. Both metalloendoproteases and serine protease activity were revealed in both EMDOGAIN and developing enamel. The roles of the amelogenin and enzyme components, if any, in periodontal regeneration are unknown.
ISSN:0300-8207
1607-8438
DOI:10.1080/03008200290000880