Emticicia sediminis sp. nov. isolated from sediment of a shallow stream

• Published in: International journal of systematic and evolutionary microbiology Vol. 65; no. 8; pp. 2496 - 2499
• Main Authors: Park, Si-Eun, Lee, Siwon, Kim, Haneul, Ahn, Tae-Young
• Format: Journal Article
• Language: English
• Published: England 01.08.2015
• Subjects: Bacterial Typing Techniques; Base Composition; Cytophagaceae - classification; Cytophagaceae - genetics; Cytophagaceae - isolation & purification; DNA, Bacterial - genetics; Fatty Acids - chemistry; Geologic Sediments - microbiology; Molecular Sequence Data; Nucleic Acid Hybridization; Phospholipids - chemistry; Phylogeny; Republic of Korea; Rivers - microbiology; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; Republic of Korea
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.000292

A novel bacterial strain, designated JBR12T, was isolated from sediment of a shallow stream in Cheonan, Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JBR12T belongs to the genus Emticicia, and indicated that its closest relatives are Emticicia oligotrophica DSM 17448T (97.8 % sequence similarity) and E. ginsengisoli Gsoil 085T (94.3%). A DNA-DNA hybridization experiment revealed < 70 % genomic relatedness between strain JBR12T and E. oligotrophica DSM 17448T. The major fatty acids (>5% of the total) were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 3-OH, anteiso-C15 : 0 and iso-C15 : 0 3-OH. The DNA G+C content of strain JBR12T was 37.7 mol%. According to data from the present polyphasic taxonomic study, strain JBR12T represents a novel species of the genus Emticicia, for which the name Emticicia sediminis sp. nov. is proposed. The type strain is JBR12T ( = KACC 17466T = JCM 19321T).