Analysis of carminomycin in human serum by fluorometric high-performance liquid chromatography

A method is given for the determination of carminomycin (CMM) and a major metabolite carminomycinol (CMMOH) in serum from cancer patients after intravenous administration of carminomycin as the free drug. CMM and CMMOH are extracted from serum with chloroform, the extract evaporated and the residue...

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Bibliographic Details
Published inJournal of chromatography Vol. 223; no. 1; pp. 155 - 164
Main Authors Fandrich, Susan E., Pittman, Kenneth A.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 10.04.1981
Subjects
Analysis of Variance
Carubicin - administration & dosage
Carubicin - blood
Carubicin - metabolism
Chromatography, High Pressure Liquid - methods
Daunorubicin - analogs & derivatives
Humans
Injections, Intravenous
Neoplasms - blood
Reference Standards
Time Factors
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Summary:A method is given for the determination of carminomycin (CMM) and a major metabolite carminomycinol (CMMOH) in serum from cancer patients after intravenous administration of carminomycin as the free drug. CMM and CMMOH are extracted from serum with chloroform, the extract evaporated and the residue dissolved in methanol. High-performance liquid chromatography analysis utilized a C 18 μBondapak reversed-phase column eluted with 0.1 mol/l acetate buffer (pH 4) — acetonitrile (60:40, v/v) with fluorescence detection. The assay is linear, reproducible, and precise with a limit of detection of 2 ng/ml. Representative serum levels of CMM and CMMOH in a cancer patient are presented.
ISSN:0378-4347
DOI:10.1016/S0378-4347(00)80078-X