Identification of the metabolites of biologically active xanthones isolated from Halenia elliptica D. Don by high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry

• Published in: Chinese chemical letters Vol. 22; no. 7; pp. 839 - 842
• Main Authors: Feng, Ru, Shi, Jian Gong, Liu, Xiao Wei, Che, Chun Tao, Yeung, John H.K., Wang, Yan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.07.2011
• Subjects: Biotransformation; Halenia elliptica; LCMS n-ESI-IT-TOF; Metabolite identification; Xanthone; 代谢产物; 氧杂蒽酮; 液相色谱分离; 生物活性; 离子阱; 花锚; 鉴定; 飞行时间质谱; Xanthone; Halenia elliptica; Metabolite identification; LCMS n -ESI-IT-TOF; Biotransformation
• ISSN: 1001-8417; 1878-5964
• DOI: 10.1016/j.cclet.2010.12.021

Metabolism study has been carried out on 1-hydroxy-2,3,5-trimethoxyxanthone （HM-1） and 1-hydroxy-2,3,4,7- tetramethoxyxanthone （HM-2）, which are two biologically active ingredients isolated from the Tibetan herb, Halenia elliptica D. Don., in rat liver microsomes in vitro. A method of high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry （LCMSn-ESI-IT-TOF） was applied to analyze metabolites of HM-1 and HM-2 on line, and five metabolites were identified containing 1,5-dihydroxy-2,3-dimethoxyxanthone （HM-5）, 1,7-dihydroxy-2,3,4-trimethoxyxanthone （HM-9）, 1,4, 7- trihydroxy-2,3-dimethoxyxanthone （HM-10）, 1,4-dihydroxy-2,3,7-trimethoxyxanthone （HM-11） and 1,2-dilaydroxy-3,4,7- trimethoxyxanthone （HM-12）. Among these metabolites, HM-9, HM-11, and HM-12 were isomers mutually. The results indicated that HM-1 and HM-2 occurred Phase I metabolic reaction of demethylation in rat microsomes in vitro.