Low coherence digital holography microscopy based on the Lorenz-Mie scattering model

We demonstrate the use of low spatial and temporal coherence holography microscopy, based on the Lorenz-Mie model, using the standard tungsten-halogen lamp present in an inverted microscope. An optical model is put forward to incorporate the effect of spectral width and different incidence angles of...

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Bibliographic Details
Published inOptics express Vol. 25; no. 21; pp. 25853 - 25866
Main Authors Vandewiele, Stijn, Strubbe, Filip, Schreuer, Caspar, Neyts, Kristiaan, Beunis, Filip
Format Journal Article
LanguageEnglish
Published United States 16.10.2017
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Summary:We demonstrate the use of low spatial and temporal coherence holography microscopy, based on the Lorenz-Mie model, using the standard tungsten-halogen lamp present in an inverted microscope. An optical model is put forward to incorporate the effect of spectral width and different incidence angles of the incident light determined by the aperture at the back focal plane of the condenser lens. The model is validated for 899 nm diameter polystyrene microspheres in glycerol, giving a resolution of 0.4% for the index of refraction and 2.2% for the diameter of the particles.
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ISSN:1094-4087
1094-4087
DOI:10.1364/OE.25.025853