Comparison of in vitro chondrogenic potential of human mesenchymal stem cells derived from bone marrow and adipose tissue

In the present work, the human bone marrow and adipose tissue-derived mesenchymal stem cells (MSCs) were isolated and expanded under in vitro condition. After their phenotypic analysis, the chondrogenic differentiation was induced by using of the three-dimensional culture system without supplementat...

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Published inGeneral physiology and biophysics Vol. 28; no. 1; p. 56
Main Authors Danisovic, Lubos, Varga, Ivan, Polák, Stefan, Ulicná, Marcela, Hlavacková, Lívia, Böhmer, Daniel, Vojtassák, Ján
Format Journal Article
LanguageEnglish
Published Slovakia 01.03.2009
Subjects
Adipose Tissue - cytology
Adipose Tissue - physiology
Adult
Adult Stem Cells - cytology
Adult Stem Cells - physiology
Antigens, CD - metabolism
Bone Marrow Cells - cytology
Bone Marrow Cells - physiology
Cell Culture Techniques
Cells, Cultured
Chondrogenesis - physiology
Collagen Type II - metabolism
HLA Antigens - metabolism
Humans
Immunohistochemistry
Male
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - physiology
Phenotype
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Summary:In the present work, the human bone marrow and adipose tissue-derived mesenchymal stem cells (MSCs) were isolated and expanded under in vitro condition. After their phenotypic analysis, the chondrogenic differentiation was induced by using of the three-dimensional culture system without supplementation of growth factors, and their chondrogenic potential was compared. Obtained results proved that both types of MSCs undergo the process of chondrogenic differentiation. Comparative analysis showed that chondrogenic potential of adipose tissue-derived MSCs was slightly decreased in comparison with bone marrow-derived MSCs. However, both evaluated MSCs may play important role in the cartilage tissue engineering.
ISSN:0231-5882
DOI:10.4149/gpb_2009_01_56