Preparation and characterizations of EGDE crosslinked chitosan electrospun membranes

• Published in: Clinical hemorheology and microcirculation Vol. 60; no. 1; pp. 39 - 50
• Main Authors: Aqil, A, Tchemtchoua, V T, Colige, A, Atanasova, G, Poumay, Y, Jérôme, C
• Format: Journal Article Web Resource
• Language: English
• Published: Netherlands IOS Press 01.01.2015
• Subjects: Bandages; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Chemistry; Chimie; chitosan; Chitosan - chemistry; Chitosan - pharmacology; electrospinning; Endothelial Cells - physiology; Engineering, computing & technology; Epoxy Resins - chemistry; Epoxy Resins - pharmacology; Fibroblasts - physiology; Humans; Ingénierie, informatique & technologie; Materials science & engineering; Materials Testing; Membranes, Artificial; Nanocomposites - chemistry; nanofiber; Nanofibers - chemistry; Physical, chemical, mathematical & earth Sciences; Physique, chimie, mathématiques & sciences de la terre; Polyethylene Glycols - chemistry; Polyethylene Glycols - pharmacology; Science des matériaux & ingénierie; Tissue Engineering; tissue engineering; Chitosan; electrospinning; crosslinking; cytotoxicity; mechanical properties
• ISSN: 1386-0291; 1875-8622
• DOI: 10.3233/CH-151930

Composite Crosslinked nanofibrous membranes of chitosan, ethylene glycol diglycidyl ether (EGDE) and polyethylene oxide was successfully prepared with bead free morphology via electrospinning technique followed by heat mediated chemical crosslinking. Architectural stability of nanofiber mat in aqueous medium was achieved by chemical crosslinking of only 1% EGDE, and tensile strength tests revealed that increasing EGDE content has considerably enhance the elastic modulus of nanofibers. The structure, morphology and mechanical properties of nanofibers were characterized by Attenuated Total Reflection-Fourier Transform Infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM) and Instron machine, respectively. Skin fibroblasts and endothelial cells showed good attachment, proliferation and viability on crosslinked electrospun membranes. The results indicate a good biocompatibility and non-toxic nature of the resulted membrane.