Selective solubilization of hemagglutinin and neuraminidase from influenza viruses

A novel approach is described for the isolation of hemagglutinin and neuraminidase from influenza viruses. In contrast to published procedures, the two glycoproteins are selectively solubilized, leaving an intact viral subparticle which contains lipid, RNA and non-glycoproteins. The pronounced diffe...

Full description

Saved in:
Bibliographic Details
Published inIntervirology Vol. 5; no. 5; p. 260
Main Author Bachmayer, H
Format Journal Article
LanguageEnglish
Published Switzerland 01.01.1975
Subjects
Cetrimonium Compounds
Glycoproteins - isolation & purification
Hemagglutinins, Viral - isolation & purification
Influenza Vaccines
Lipids - analysis
Neuraminidase - isolation & purification
Orthomyxoviridae - analysis
Orthomyxoviridae - immunology
Orthomyxoviridae - ultrastructure
RNA, Viral - analysis
Solubility
Surface-Active Agents
Viral Proteins - analysis
Viral Proteins - isolation & purification
Online AccessGet more information

Cover

More Information
Summary:A novel approach is described for the isolation of hemagglutinin and neuraminidase from influenza viruses. In contrast to published procedures, the two glycoproteins are selectively solubilized, leaving an intact viral subparticle which contains lipid, RNA and non-glycoproteins. The pronounced differences in size allow a simple separation of the solubilized proteins from the virus 'core'.
ISSN:0300-5526
DOI:10.1159/000149923