Ioxynil and diethylstilbestrol increase the risks of cardiovascular and thyroid dysfunction in zebrafish

• Published in: The Science of the total environment Vol. 838; no. Pt 3; p. 156386
• Main Authors: Li, Yi-Feng, Rodrigues, Joana, Campinho, Marco A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10.09.2022
• Subjects: Diethylstilbestrol; Endocrine disruption; Heart; Ioxynil; Thyroid; Vascular; Heart; Endocrine disruption; Diethylstilbestrol; Vascular; Ioxynil; Thyroid
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2022.156386

Endocrine disruption results from exposure to chemicals that alter the function of the endocrine system in animals. Chronic 60 days of exposure to a low dose (0.1 μM) of ioxynil (IOX) or diethylstilbestrol (DES) via food was used to determine the effects of these chemicals on the physiology of the heart and thyroid follicles in juvenile zebrafish. Immunofluorescence analysis and subsequent 3D morphometric analysis of the zebrafish heart revealed that chronic exposure to IOX induced ventricle deformation and significant volume increase (p < 0.001). DES exposure caused a change in ventricle morphology, but volume was unaffected. Alongside, it was found that DES exposure upregulated endothelial related genes (angptl1b, mhc1lia, mybpc2a, ptgir, notch1b and vwf) involved in vascular homeostasis. Both IOX and DES exposure caused a change in thyroid follicle morphology. Notably, in IOX exposed juveniles, thyroid follicle hypertrophy was observed; and in DES-exposed fish, an enlarged thyroid field was present. In summary, chronic exposure of juvenile zebrafish to IOX and DES affected the heart and the thyroid. Given that both chemicals are able to change the morphology of the thyroid it indicates that they behave as endocrine disruptive chemicals (EDCs). Heart function dynamically changes thyroid morphology, and function and hence it is likely that the observed cardiac effects of IOX and DES are the source of altered thyroid status in these fish. [Display omitted] •60-day exposure to IOX “safe levels” induced ventricle deformation in zebrafish.•IOX exposure caused thyroid follicle hypertrophy in zebrafish juveniles.•DES-exposure caused abnormal ventricle morphology without volume change.•DES exposition altered vascular gene expression and enlarged thyroid gland field.•IOX and DES present EDC potential on the thyroid axis at “safe levels”.