Isolation of bovine cardiodilatin by fast protein liquid chromatography and reversed-phase high-performance liquid chromatography

Cardiodilatin (CDD), a polypeptide exhibiting vasorelaxant and diuretic natriuretic bioactivity, was isolated from bovine atria. The isolation procedure reported here is different from that originally used for the purification of porcine and bovine CDD. Instead of cation-exchange chromatography on F...

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Bibliographic Details
Published inJournal of chromatography Vol. 397; p. 347
Main Authors Hock, D, Schriek, U, Fey, E, Forssmann, W G, Mutt, V
Format Journal Article
LanguageEnglish
Published Netherlands 26.06.1987
Subjects
Amino Acid Sequence
Animals
Atrial Natriuretic Factor
Cattle
Chromatography, High Pressure Liquid
Hydrolysis
Muscle Proteins - analysis
Muscle Proteins - isolation & purification
Muscle, Smooth, Vascular - drug effects
Myocardial Contraction - drug effects
Myocardium - analysis
Spectrophotometry, Ultraviolet
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Summary:Cardiodilatin (CDD), a polypeptide exhibiting vasorelaxant and diuretic natriuretic bioactivity, was isolated from bovine atria. The isolation procedure reported here is different from that originally used for the purification of porcine and bovine CDD. Instead of cation-exchange chromatography on Fractogel TSK-CM 650 S and several purification steps on different high-performance liquid chromatographic (HPLC) columns, it is now possible to obtain CDD-88 by an automated fast-protein liquid chromatography system for repeated injections and a motor valve as fraction collector and only one final step of reversed-phase HPLC on a TSK-ODS-120T column.
DOI:10.1016/S0021-9673(01)85018-4