Histamine-containing cells from bronchial lavage of macaque monkeys. Time course and inhibition of anaphylactic histamine release

Bronchial lavage of rhesus and cynomolgus monkeys provided leucocyte suspensions with viable histamine-containing cells (HCC) as 1--8% of the white cell population. These HCC released histamine or challenge with antiserum to human IgE. HCC from 2 monkeys with pulmonary and cutaneous hypersensitivity...

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Published inInternational archives of allergy and applied immunology Vol. 62; no. 2; p. 205
Main Authors Butchers, P R, Vardey, C J, Skidmore, I F, Wheeldon, A, Boutal, L E
Format Journal Article
LanguageEnglish
Published Switzerland 01.01.1980
Subjects
Albuterol - pharmacology
Anaphylaxis - immunology
Animals
Ascaris - immunology
Cromolyn Sodium - pharmacology
Female
Haplorhini
Histamine
Histamine Release - drug effects
Isoproterenol - pharmacology
Lung - cytology
Macaca fascicularis
Macaca mulatta
Macrophages
Male
Therapeutic Irrigation
Time Factors
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Summary:Bronchial lavage of rhesus and cynomolgus monkeys provided leucocyte suspensions with viable histamine-containing cells (HCC) as 1--8% of the white cell population. These HCC released histamine or challenge with antiserum to human IgE. HCC from 2 monkeys with pulmonary and cutaneous hypersensitivity to Ascaris antigen (AA) released histamine on challenge with AA. The extent of histamine release was related to the concentration of antigen and antiserum, and histamine release was complete within 10 min of challenge. (+/-)Salbutamol and (-)isoprenaline were potent inhibitors of anaphylactic histamine release from HCC, but disodium cromoglycate and AH 9679 were relatively poor inhibitors. The HCC system combines the reproducibility of a cell suspension with a response to drugs similar to that of human lung fragments.
ISSN:0020-5915
DOI:10.1159/000232513