Fecal Genetic Mutations and Human DNA in Colorectal Cancer and Polyps Patients
Colorectal cancer (CRC) is one of the most frequent cancers. Genetic mutations in CRC already described can be detected in feces. Microarray methods in feces can represent a new diagnostic tool for CRC and significant improvement at public health. to analyze stool DNA by human DNA quantify and micro...
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Published in | Asian Pacific journal of cancer prevention : APJCP Vol. 20; no. 10; pp. 2929 - 2934 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Thailand
West Asia Organization for Cancer Prevention
01.10.2019
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Subjects | |
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Abstract | Colorectal cancer (CRC) is one of the most frequent cancers. Genetic mutations in CRC already described can be detected in feces. Microarray methods in feces can represent a new diagnostic tool for CRC and significant improvement at public health.
to analyze stool DNA by human DNA quantify and microarray methods as alternatives to CRC screening.
Three methods were analyzed in stool samples: Human DNA Quantify, RanplexCRC and KRAS/BRAF/PIK3CA (KBP) Arrays.
KBP array mutations were presented in 60.7% of CRC patients and RanplexCRC Array mutations in 61.1% of CRC patients. Sensitivity and specificity for human DNA quantification was 66% and 82% respectively. Fecal KBP Array had 35% sensitivity and 96% specificity and RanplexCRC Array method had 78% sensitivity and 100% specificity.
Microarray methods showed promise as potential biomarkers for CRC screening; however, these methods had to be optimized to improve accuracy and applicability by clinical routine. |
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AbstractList | Colorectal cancer (CRC) is one of the most frequent cancers. Genetic mutations in CRC already described can be detected in feces. Microarray methods in feces can represent a new diagnostic tool for CRC and significant improvement at public health.
to analyze stool DNA by human DNA quantify and microarray methods as alternatives to CRC screening.
Three methods were analyzed in stool samples: Human DNA Quantify, RanplexCRC and KRAS/BRAF/PIK3CA (KBP) Arrays.
KBP array mutations were presented in 60.7% of CRC patients and RanplexCRC Array mutations in 61.1% of CRC patients. Sensitivity and specificity for human DNA quantification was 66% and 82% respectively. Fecal KBP Array had 35% sensitivity and 96% specificity and RanplexCRC Array method had 78% sensitivity and 100% specificity.
Microarray methods showed promise as potential biomarkers for CRC screening; however, these methods had to be optimized to improve accuracy and applicability by clinical routine. |
Author | Deak, Elisabeth Pimenta, Célia Lima, Jacqueline Saad, Sarhan Sydney Teixeira, Yolanda Murray, Helena Silva, Tiago Donizetti Manoukian Forones, Nora Junior, Ermelindo Ermelindo Della Libera Felipe, Aledson Vitor |
AuthorAffiliation | 1 Oncology Group, Gastroenterology Division, Medicine Department 3 Surgery Department, Universidade Federal de Sao Paulo, São Paulo, Brazil 2 Gastroenterology Division and Endoscopist 4 Randox Laboratories Ltd., Crumlin, Co. Antrim, United Kingdom |
AuthorAffiliation_xml | – name: 3 Surgery Department, Universidade Federal de Sao Paulo, São Paulo, Brazil – name: 1 Oncology Group, Gastroenterology Division, Medicine Department – name: 2 Gastroenterology Division and Endoscopist – name: 4 Randox Laboratories Ltd., Crumlin, Co. Antrim, United Kingdom |
Author_xml | – sequence: 1 givenname: Jacqueline surname: Lima fullname: Lima, Jacqueline organization: Oncology Group, Gastroenterology Division, Medicine Department, Universidade Federal de Sao Paulo, São Paulo, Brazil – sequence: 2 givenname: Yolanda surname: Teixeira fullname: Teixeira, Yolanda organization: Oncology Group, Gastroenterology Division, Medicine Department, Universidade Federal de Sao Paulo, São Paulo, Brazil – sequence: 3 givenname: Célia surname: Pimenta fullname: Pimenta, Célia organization: Oncology Group, Gastroenterology Division, Medicine Department, Universidade Federal de Sao Paulo, São Paulo, Brazil – sequence: 4 givenname: Aledson Vitor orcidid: 0000-0003-1335-1478 surname: Felipe fullname: Felipe, Aledson Vitor organization: Oncology Group, Gastroenterology Division, Medicine Department, Universidade Federal de Sao Paulo, São Paulo, Brazil – sequence: 5 givenname: Tiago Donizetti surname: Silva fullname: Silva, Tiago Donizetti organization: Oncology Group, Gastroenterology Division, Medicine Department, Universidade Federal de Sao Paulo, São Paulo, Brazil – sequence: 6 givenname: Ermelindo Ermelindo Della Libera surname: Junior fullname: Junior, Ermelindo Ermelindo Della Libera organization: Gastroenterology Division and Endoscopist, Universidade Federal de Sao Paulo, São Paulo, Brazil – sequence: 7 givenname: Sarhan Sydney surname: Saad fullname: Saad, Sarhan Sydney organization: Surgery Department, Universidade Federal de Sao Paulo, São Paulo, Brazil – sequence: 8 givenname: Elisabeth surname: Deak fullname: Deak, Elisabeth organization: Surgery Department, Universidade Federal de Sao Paulo, São Paulo, Brazil – sequence: 9 givenname: Helena surname: Murray fullname: Murray, Helena organization: Randox Laboratories Ltd., Crumlin, Co. Antrim, United Kingdom – sequence: 10 givenname: Nora surname: Manoukian Forones fullname: Manoukian Forones, Nora organization: Oncology Group, Gastroenterology Division, Medicine Department, Universidade Federal de Sao Paulo, São Paulo, Brazil |
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Keywords | Screening colorectal cancer Stool DNA Polyps colonoscopy |
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Snippet | Colorectal cancer (CRC) is one of the most frequent cancers. Genetic mutations in CRC already described can be detected in feces. Microarray methods in feces... |
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SubjectTerms | Aged Biomarkers, Tumor - genetics Case-Control Studies Colonic Polyps - diagnosis Colonic Polyps - genetics Colonoscopy Colorectal Neoplasms - diagnosis Colorectal Neoplasms - genetics DNA, Neoplasm - analysis DNA, Neoplasm - genetics Early Detection of Cancer - methods Feces - chemistry Female Follow-Up Studies Humans Male Middle Aged Mutation Prognosis Proto-Oncogene Proteins B-raf - genetics Proto-Oncogene Proteins p21(ras) - genetics |
Title | Fecal Genetic Mutations and Human DNA in Colorectal Cancer and Polyps Patients |
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