Fecal Genetic Mutations and Human DNA in Colorectal Cancer and Polyps Patients

• Published in: Asian Pacific journal of cancer prevention : APJCP Vol. 20; no. 10; pp. 2929 - 2934
• Main Authors: Lima, Jacqueline, Teixeira, Yolanda, Pimenta, Célia, Felipe, Aledson Vitor, Silva, Tiago Donizetti, Junior, Ermelindo Ermelindo Della Libera, Saad, Sarhan Sydney, Deak, Elisabeth, Murray, Helena, Manoukian Forones, Nora
• Format: Journal Article
• Language: English
• Published: Thailand West Asia Organization for Cancer Prevention 01.10.2019
• Subjects: Aged; Biomarkers, Tumor - genetics; Case-Control Studies; Colonic Polyps - diagnosis; Colonic Polyps - genetics; Colonoscopy; Colorectal Neoplasms - diagnosis; Colorectal Neoplasms - genetics; DNA, Neoplasm - analysis; DNA, Neoplasm - genetics; Early Detection of Cancer - methods; Feces - chemistry; Female; Follow-Up Studies; Humans; Male; Middle Aged; Mutation; Prognosis; Proto-Oncogene Proteins B-raf - genetics; Proto-Oncogene Proteins p21(ras) - genetics; Screening; colorectal cancer; Stool DNA; Polyps; colonoscopy
• ISSN: 1513-7368; 2476-762X
• DOI: 10.31557/APJCP.2019.20.10.2929

Colorectal cancer (CRC) is one of the most frequent cancers. Genetic mutations in CRC already described can be detected in feces. Microarray methods in feces can represent a new diagnostic tool for CRC and significant improvement at public health. to analyze stool DNA by human DNA quantify and microarray methods as alternatives to CRC screening. Three methods were analyzed in stool samples: Human DNA Quantify, RanplexCRC and KRAS/BRAF/PIK3CA (KBP) Arrays. KBP array mutations were presented in 60.7% of CRC patients and RanplexCRC Array mutations in 61.1% of CRC patients. Sensitivity and specificity for human DNA quantification was 66% and 82% respectively. Fecal KBP Array had 35% sensitivity and 96% specificity and RanplexCRC Array method had 78% sensitivity and 100% specificity. Microarray methods showed promise as potential biomarkers for CRC screening; however, these methods had to be optimized to improve accuracy and applicability by clinical routine.